Mon. 3-29 - Changed Human Brain Endothelial Cell (HBMEC) coverslips to minimal media. Handed on to Aline for PI treatment. Will stain with H2DCFDA for confocal imaging on 3-31.
Plated 24-well plate, with coverslips, of HT22 cells for Tat/Meth treatment and H2DCFDA stain.
Plated new 24-well plate (no coverslips) of HBMEC for Monica's repeat of Methamphetemine toxicity study. Will supervise treatment on 3-30.
Passed Human Umbilical Vein Endothelial Cells (HUVEC). Split one flask into three.
Drove to PetSmart. Purchased new litter tray (with odor-absorbing cover), litter-catching mat, rubber tray to go under water dish, and 8lb of IAMS light hairball control formula.
Took 52-E to 805-S to 163-S to Friar's Road. Visited The Container Store to purchase plastic shoeboxes, cedar blocks for clothes storage, a large container for cat food, and a pan-lid rack for the kitchen. Also obtained an acid-free archival quality box. Box will be used to store wedding dress, which is to be picked up sometime before 4-2.
Returned home, met with building manager to establish protocol for our early vacation of premesis, and transferring apartment to two post doctoral students from London.
Dinner was made by Matt, the popular combination of Trader Joe's Marinara, Hunt's Tomato Sauce, 95% lean ground beef, and various spices, poured over al dente pasta.
Tues. 3-30 - Blocked PGP test blots for 1hr @ room temp in 5% non-fat milk solution. Washed 4 times in PBS-T. Put in primary Ab solution at the following concentrations: 1:250, 1:500, 1:1000, 1:2500. Incubated overnight in cold room.
Walked to bank, withdrew $20 for gas money.
Returned to lab to eat lunch.
Wrote rather silly and rambling blog entry, in the process of which remembering what I have left to do today...followed by publishing and getting on with the day's work.